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KMID : 0894319970060010177
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Dongguk Journal of the Institute of Oriental Medicine
1997 Volume.6 No. 1 p.177 ~ p.203
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A study on screeining of antibacterial herb medicines against puerperal infecting-bacteria, Proteus vulgaris, Staphylococcus aures, and Enterococcus faecalis
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Abstract
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Various kinds of medicinal herbs and prescribed herb medicines which have usually been used for treatment of reducing fever, purging intense heat, and detoxication were secreened for rial activity against Proteus vulgaris, S. aureus, and E. faecalis which causing puerperal infection. Minimal inhibitory concentrations of water- and ethanol-soluble extracts of Hwangyon(C. japonica), Omae(P. mume), and Omiza(S. chinensis) were determined and using thin-layer chromatography the extracts of Hwangyon and Omiza were separated. The results obtained were as follows :
1. The water-soluble extracts of Keumeunhwa(L. japonica), Chiza(G. jasminoides), Hwangyon(C. japonica), Dansam(S. miltiorrhiza), Omae(P. mume) and Omiza(S. chinensis) showed antibacterial activities against P. vulgaris ; among them, the extracts of Hwangyon, Omae, and Omiza showed high antibacterial activities. The water-soluble extracts of Keumeunhwa(L. japonica), Hwangyon(C. japonica), Hwangkeum(S. baicalensis), Hwangbaek(P. amurense), Dansam(S. miltiorrhiza), Daehwang(R. coreanum), Omae(P. mume), Omiza(S. chinensis), Gingseng(P. ginseng), and Gamcho(G. uralensis) showed antibacterial activities against S. aureus ; among them, the extracts of Hwangyon and Omiza showed high antibacterial activities. The water-soluble extracts of Banggi(C. trilobus), Daehwang(R. coreanum), Omae(P. mume), Omiza(S. chinensis), and Gamcho(G. uralensis) inhibited the cell growth of E. faecalis ; among them, Omae and Omiza showed high antibacterial activities. The extract of Hwangyon(C. japonica) and Omae(P. mume) showed no inhibition against E. coli. However, the extract of Omiza(S. chinensis) inhibited cell growth of both E. coli and B. subtilis.
2. The water- and ethanol-soluble extract of Hwangyon(C. japonica) showed antibacterial activities against P. vulgaris and S. aureus, those of Omae(P. mume) against P. vulgaris and E. faecalis, and those of Omiza(S. chinensis) against all species tested, P. vulgaris, S. aureus, and E. faecalis. With the exception that ethanol-soluble extract of Hwangyon(C. japonica) showed much higher antibacterial activity against S. aureus than water-soluble one, antibacterial activities of both water-and ethanol-soluble ones were similar to each other of other two medicinal herbs.
3. When the prescribed herb medicines were tested, Sambohwan showed antibacterial activities against P. vulgaris and S. aureus. No prescribed herb medicine inhibitied the cell growth of E. faecalis.
4. Minimal inhibitory concentrations(MICs) of both water- and ethanol-soluble extracts of Hwangyon(C. japonica), Omae(P. mume), and Omiza(S. chinensis) against P. vulgaris were 25mgM, 10m91W, and 20m9/W, respectively. Those of both Hwangyon(C. japonica) and Omiza(S. chinensis) against S. aureus were 1.25m9/m~ and 10mg1mg, respectively. MICs of water-soluble extracts of Omae (P. mume) and Omiza(S. chinensis) against E. faecalis were 2.5m9/m2 and 5m91W, and those of ethanol-soluble extracts were 5m9/mQ and 10m9/d, respectively. Except for those of E. faecalis, the cell growth of P. vulgaris and S. aureus were inhibited by much lower concentration of ethanol-soluble extracts used.
As a result, the antibacterial compounds against P. vulgaris, S. aureus, and E. aecalis are contained in the_ extracts of Hwangyon(C. japonica), Omae(P. mume), Omiza(S. chinensis), the prescribed herb medicine, Sambohwan, and might be used for treatment of puerperal fi fection.Further study should be carried out to identify which compounds affect the cell growth inhibition of P. vulgaris, S. aureus, and E. faecalis.
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